Fig. 3

MS-275 enhances NK cell-mediated cytotoxicity against DMG cells. A-B Luciferase-engineered TT150630 and TT190326 cell lines were treated with DMSO or 1µM MS-275 for 2 days and cocultured with NK-92MI cells at the indicated E:T ratios. After 4 h of incubation, bioluminescence was measured, and NK cell-induced cytotoxicity (%) was calculated and plotted. C-F DMG cells were treated with MS-275 (1µM) for 2 days, stained with calcein AM, and cocultured with NK-92MI cells in 96-well plates at a 1:1 ratio (TT190326, TT150630 and TT170720) or a 5:1 ratio (TT150728). After incubation for 4 h, fluorescence images were captured using an inverted microscope. Images of DMG cell lines showing a reduction in fluorescence (indicating cell death) are presented (left). Calcein AM-stained DMG cells without NK-92MI cells served as controls. The percentage (%) of cells undergoing NK cell-induced cytotoxicity was calculated and plotted (right). Statistical significance was assessed via Student’s t test, with *p < 0.05 and **p < 0.01. Scale bars, 500 μm