Fig. 5

(A) Approaches to analyse copy number alterations, here exemplified for measuring chromosome 3p loss. (B) Simulated and observed copy number value measurements for chromosome 3p in vfDNA-41, with associated 99%-confidence intervals. Simulations were based on the initial Gα_q mutant and wild-type measurements and the assumption of a clonal loss in the vfDNA melanoma-cell population, in single experiments using both the classic and SNP-based approach. Significance of the measurements is indicated by the symbol and colour of the individual confidence interval (empty symbol and red colour: not significantly different from 2 [p > 0.01], filled symbol and grey colour: significantly different from 2 [p < 0.01]). (C) Overview of simulated and observed copy number value measurements for chromosome 3p in vfDNA-24, in both single and (3x) merged experiments using the SNP-based approach. (D) Overview of simulated copy number value measurements for chromosome 3p in vfDNA-19, in both single and (3x) merged experiments using the SNP-based approach.