Fig. 7
B16 melanoma IRF1+IRF2 double KO cells show impaired responses to IFN inducer Poly(I:C) plus CPI. A NSG mice (n=10) and C57BL/6 mice (n=12) were subcutaneously injected with WT, IRF2KO and IRF1+IRF1KO cells and data display tumor growth in individual mice. B Tumors were collected on day 15 and the surface MHC I expression of C57BL/6 tumors was analyzed by flow cytometry. C C57BL/6 mice (n=45), that were treated with poly(I:C) and aPD1 were injected with IFNα treated (10ng/mL, 24h) control WT vs IRF2KO IRF2+IRF1 (double) KO B16 cells and tumor growth was followed. D & E On day 17 post tumor injection, n=5 mice/group were sacrificed for: D mRNA expression analysis of MHC I pathway components using qPCR method and E the tumor cell surface expression analysis of MHC I molecules by flow cytometer. Each dot represents the average measurement of the individual tumors collected from mice. F Survival analysis of WT or IRF2KO or IRF1+IRF2 KO tumors in C57BL/6 mice. This experiment was repeated twice. Tumor growth curve shows mean+SD (C)