Fig. 5

Effect of IFNα on WT and IRF2 KO B16 melanomas. A MHC I levels on IRF2KO (n=3) and WT cells (n=3) after 24 hour stimulation with or without IFNα (histograms) and after withdrawal of IFN (line graph). B mRNA expression of MHC I pathway components in WT (n=2) and IRF2KO cells (n=2) after the IFN treatments. C Diagram of the experiment testing the effects of IFNα+poly(I:C) treatment ± anti-PD1 on WT vs IRF2KO B16 melanoma. D & E Day 15 post tumor injection, tumors were harvested and (D) mRNA expression of the MHC I pathway components was analyzed by qPCR and E the expression of MHC I molecules on the tumors was analyzed by flow cytometer. Each dot represents a biological replicate and the curves on A. and C. show mean+SD. F Another group of C57BL/6 mice (n=40) were injected with WT (n=20) and IRF2KO (n=20) tumors for survival and tumor growth was recorded for survival analysis. Statistical analysis was calculated by GraphPad Prism, **P < 0.01, ***P < 0.001