Fig. 3

Loss of IRF2 in the mouse melanoma cell line B16F0 reduces the expression of MHC I pathway components but has no effect on B16F0 tumor growth kinetics in NSG or C57BL/6 mice. A Diagram of the experimental setup and B In vitro mRNA expression levels of MHC I pathway components in B16F0 WT (n=4) vs IRF2KO (n=3) were analyzed by qPCR. C Diagram of experiments testing the in vivo growth of WT vs IRF2KO B16F0 cells in NSG mice (n=10) and C57BL/6 mice (n=10) D & E Tumors from C were collected on day 15 and MHC I expression was analyzed by flow cytometry (D) and mRNA expression of the MHC I pathway components was analyzed by qPCR (E). Each dot represents a biological replicate and the curves on F show mean +SD. Statistical analysis was calculated by GraphPad Prism, **P < 0.01, ***P < 0.001